The Indian Journal of Genetics and Plant Breeding

SCOPUSWeb of Science
  • Year: 2005
  • Volume: 65
  • Issue: 3

Diversity analysis by RAPD and ISSR markers among the selected mungbean [Vigna radiata (L.) Wilczek] genotypes

  • Author:
  • K. Chattopadhyay, Md. Nasim Ali, H. K. Sarkar, N. Mandai, S. Bhattacharyya
  • Total Page Count: 3
  • DOI:
  • Page Number: 173 to 175

Dept. of Genetics and Biotechnology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia 741 252.

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Abstract

Narrow genetic base in the working collection of mungbean in India is a bottleneck in the improvement of this crop. Present study gave emphasis for making sizable, but effective working gene pool. Molecular markers, like RAPD and ISSR were employed to assess the genetic diversity among selected germplasm comprising varieties, landraces and wild accessions. Though polymorphism among the varieties was moderate, it was high (83%) when the whole set of germplasm was considered. One mungbean variety, PS-16 with determinate growth habit and a wild accession, Sub-14 (V. radiata var. sublobata) was found most diverse as revealed from the lowest Jaccard's similarity coefficient value (0.34). One of the landraces from West Bengal, A-5 (Chinnimung) was also found distantly located in dendrogram from all varieties and other landraces. The efficiency of ISSR markers over RAPD markers was well visualized from higher frequency polymorphic bands and polymorphic information content values.

Keywords

Mungbean, RAPD, ISSR markers, genetic diversity