Directorate of Wheat Research, Regional Station, Flowerdale, Shimla 171 002.
1Present address: Indian Institute of Vegetable Research, P.B. # 5002, Gandhi Nagar, Naria, Varanasi 221 005.
Molecular markers for the leaf rust resistance genes Lr9, Lr19, Lr24, rye specific chromatin (1BL.1RS translocation, Lr26), and Lr28 were validated in the parental lines of wheat (Triticum aestivum L.). The markers for Lr9, Lr19 and Lr26 were able to discriminate the lines with specific genes/chromatin from the lines that did not carry these genes. The Lr24 marker showed polymorphism between positive and negative controls but in white grained genotypes the amplified band was faint and inconsistent as compared to red seeded lines. The marker used for detecting Lr28 was not polymorphic among the tested carrier and non-carrier lines. The markers of Lr19 and Lr24 were utilized for authenticating the presence of specific genes in the advanced breeding lines whereas rye-chromatin specific marker was used for marker assisted selection of Lr26. Genetic stocks with combined resistance genes Lr19 + Lr26 + 5r25 + 5r31 + Yr9 + Yr27 and Lr24 + Lr26 + 5r24 + 5r31 + Yr9 + Yr27 were developed in the background of PBW 343 with the aid of pedigree information, host-pathogen interaction and molecular marker.
Wheat, leaf rust resistance, molecular markers, gene pyramiding, marker validation
