1 Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005, India.
2 Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005, India.
* Author for correspondence; email: joshi_vns@yahoo.co.in
Spot blotch of barley caused by Cochliobolus sativus is an important foliar disease of barley causing considerable yield loss every year. The present study was undertaken to identify molecular markers for the locus controlling spot blotch resistance in the accession IBON 18, using a set of 110 recombinant inbred lines (RILs). The screening of individual RILs using an isolate highly virulent on the popular Indian cultivar ‘RD 2508’ revealed the presence of a major locus for spot blotch resistance in IBON 18. Based on the screening of 360 RAPD primers employing Bulk Segregant Analysis (BSA), 75 (20.83%) primers gave polymorphic bands in between parents. Out of these, two RAPD markers OPM04 625 and OPB01520 were found to be linked to the spot blotch resistance locus with a map distance of 6.5 and 10.9 cM, respectively. The linked markers appear to be useful in incorporating spot blotch resistance gene into barley breeding lines.
Hordeum vulgare, bulked segregant analysis, spot blotch, resistance, random amplified polymorphic DNA marker, barley
