1Department of Plant Sciences, University of Hyderabad, Gachibowli, 500 046.
2Division of Genetics and National Phytotron Facility, Indian Agricultural Research Institute, New Delhi, 110 012.
*Corresponding author's E-mail: rmsl@uohyd.ernet.in or mragiba@gmail.com
To detect a set of RAPD markers associated with spot blotch/Helminthosporium leaf blight (HLB) resistance in wheat, F2 population of a cross, WH 147 x Chirya 3, comprising 332 individuals was analyzed by Bulked segregant analysis (BSA). Four putative polymorphic and reproducible RAPD markers; OPK06791, OPA101040, OPN15765 and OPA12464 were identified. The markers, OPK06791 and OPA101040 were associated with resistance in repulsion phase while, OPN15765 and OPA12464 in coupling phase. Together, these markers determined a significant proportion of total phenotypic variation (25.05%). Regression analysis revealed three markers, OPK06791, OPA101040 and OPN15765 to be significantly associated with disease phenotype.
Helminthosporium leaf blight, spot blotch, RAPD markers, bulked segregant analysis, disease resistance
