The Indian Journal of Genetics and Plant Breeding

SCOPUSWeb of Science
  • Year: 2010
  • Volume: 70
  • Issue: 1

Micropropagation of Mangifera indica L. cv. Kurakkan through somatic embryogenesis

  • Author:
  • Maneesh Mishra1,, Yukti Shree1, Rajesh Pati1, Shubhendu Seal1, Neelam Shukla1, Madhu Kamle1, Ramesh Chandra1, Alka Srivastava1,2
  • Total Page Count: 6
  • DOI:
  • Page Number: 85 to 90

1Biotechnology Laboratory, Division of Crop Improvement, Central Institute for Sub-tropical Horticulture, Rehmankhera, P.O. Kakori, Lucknow, 227 107.

2Department of Botany, Lucknow University, Lucknow, 226 001.

Abstract

Nucellar embryogenesis was induced in Mangifera indica L. cv. Kurakkan, a polyembryonic salt tolerant, dwarfing rootstock. Nucellus tissue excised from 3.5 cm long fruits developed pro-embryonic callus in 19 days of inoculation on modified MS medium supplemented with 4.52μM 2,4-D, 0.05% malt extract and 13.78μM spermidine. Somatic embryogenesis exhibited high frequency (158.33 embryos). However, all the differentiated embryos proliferated on medium having low level of sucrose (4% w/v) and auxin (2.26μM 2,4-D). Most of the proembryonic calli converted into heart shaped and cotyledonary embryos by reducing temperature to 15°C. Somatic embryos were matured on modified MS medium fortified with 0.38μM ABA, 0.57μM IAA and 30.30μM PEG. Matured somatic embryos germinated (around 30%) on MS medium supplemented with 2.68μM NAA, 11.60μM kinetin and 2736.9μM glutamine.

Keywords

Abiotic stress, Kurakkan, Mangifera indica, Polyembryonic, Rootstock, Salt tolerant, Somatic embryogenesis