Breeding of black gram genotypes for resistance to bruchid beetle through molecular and conventional approaches

Vigna mungo var. silvestris (Trombay wild urd (INGR10133)) collected from Trombay hilly areas has been shown to have bruchid resistance. Recombinant inbred lines (RILs) were generated by crossing V. mungo var. mungo (cv. TU 94-2, bruchid susceptible) and V. mungo var. silvestris (bruchid resistant). A genetic linkage map constructed using 104 recombinant inbred line (RIL) in F9 generation with 428 markers (86 RAPD, 47 SSR, 41 ISSR and 254 AFLP) was used for quantitative trait loci (QTL) detection. The RILs exhibited a high level of variation for percentage of adult emergence (0–100%) and developmental period (0–105 days). Two QTLs Cmrae\A and Cmrae1.2 were identified for percentage of adult emergence on the linkage group (LG) 3 and 4 respectively. For developmental period, six QTLs were identified with two QTLs (Cmrdp\A and Cmrdp\JZ) on LG 1, three QTLs (Cmrdp\. 3 Cmrdp 1.4 and Cmrdp\. 5) on LG 2 and one QTL (Cmrdp1.6) on LG 10. DNA markers linked to bruchid resistance will aid in marker assisted selection for transfer of this trait into different genetic background. Selections from the cross between TU 94-2 and V. mungovar. silvestris has led to the development of new black gram genotypes TU-26,TU-40andTU-72 with resistance to bruchids, which are under different stages of testing.