Agrobacterium-mediated genetic transformation of pigeonpea [Cajanus cajan (L.) Millisp] for pod borer resistance: Optimization of protocol

Pod borer is a serious pest of pigeonpea. It was observed that borer infected flower buds do not produce any seed but only pods. In the present investigation, successful transfer and expression of cryIIAa gene was made through Agrobacterium into a popular pigeonpea variety, Konkan Tur-1. of pigeonpea through -mediated gene transfer method. The ability to regenerate in vitro as well use of positive selection system may provide means for recovery of transgenic pigeonpea.