Assessment of fertility restorer gene (R_f) in R-line and Moricandia based hybrid of Indian mustard using SCAR marker

Sequence characterized amplified region (SCAR) markers were developed for fertility restorer gene that is useful for hybrid development in Indian mustard.Validation of primer (SCAR-3) associated with fertility restorer gene was tested in 50 restorer inbred plants (R- line) and their F1 derived from Moricandia CMS.This was further confirmed through observation of flowers, pollen fertility/sterility and seed set. The SCAR-3 produced a sharp band (200 bp) in restorer line and their respective hybrids.The genetic purity of the hybrid was above 90% while the restorer line showed higher impurity (36%). The results showed very high correspondence between the flower, pollen fertility/sterility and seed set with SCAR marker indicating potentiality of case specific utilization of this marker in testing hybrid seed purity.