1Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing-210095, China
Horticultural Department, Nanjing Agricultural University, Nanjing 210095, China; State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing, 210095, China
*Corresponding author's e-mail: yingli@njau.edu.cn
Online published on 15 November, 2013.
A set of 24 Doubled haploid (DH) lines of non-heading Chinese cabbage obtained through microspore culture were used for identification of self-incompatibility (SI) and S haplotypes using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis with S-locus receptor kinase (SRK)-specific primers.The DH lines were grouped into five S haplotypes. The SI of two of these haplotypes was strong, whereas that of the remaining haplotypes was weak. The results obtained were consistent with the self-compatibility indices found. PCR-RFLP analysis using DH lines could enhance selection efficiency and improve the application of SI in the hybrid breeding of non-heading Chinese cabbage.
Non-heading Chinese cabbage, doubled haploid, self-incompatibility, S-locus receptor kinase (SRK), PCR-RFLP
