Finger printing and purity testing of rice hybrids using microsatellite markers

Four rice hybrids viz., KRH-2, Sahyadri, Indira Sona and Pusa RH-10 along with their parental lines were tested for genetic purity and their identity using microsatellite markers. Out of 53, eight microsatellite markers distributed on six chromosomes showed polymorphism between the parental lines of one or more hybrids. The highest frequency of polymorphism was observed in KRH-2, which exhibited distinguishing banding pattern for markers, RM9, RM17, RM212, RM256, RM335, RM444 and RM1384. Hybrid Sahyadri exhibited polymorphism for RM260 and RM444, whereas, Indira Sona showed differentiating banding pattern for RM260 and RM1384. Similarly, Pusa RH-10 exhibited the polymorphic banding pattern on five microsatellite loci i.e., RM17, RM212, RM335, RM444 and RM1384.