1Department of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut-250 110
2Department of Biosciences and Biotechnology, Banasthali Vidyapeeth, Rajasthan
3Department of Agriculture and Food Systems, Melbourne School of Land and Environment, The University of Melbourne, Victoria, Australia-3010
4Department of Plant Bioscience, Pusan National University, Miryang, Korea
5Department of Biotechnology, B.C.M.&T., C.C.S.University, Meerut 250 110
6Division of Genetics, Indian Agricultural Research Institute, New Delhi 110 012
*Corresponding author's e-mail: rajendrak64@yahoo.co.in
Online published on 16 June, 2014.
Microsatellites have become a marker of choice in plant genetics and breeding research due to their unilocus, multiallelic and highly reproducible nature. In present study EST sequences isolated from chickpea root under water stress were assessed for the frequency, type and distribution of SSR motifs. Out of the 6400 ESTs (accession no. CDO 38847 to GR 394575), SSR sequences were identified in 348 singleton contigs and from these, 50 flanking primer pairs were designed. Out of these, 41 amplified a marker of the expected size range across nineteen diverse chickpea accessions. 11 of the primer pairs were size polymorphic with an average PIC value of 0.59 across the nineteen genotypes assessed.
Chickpea, EST, microsatellites, drought tolerance
