Indian Journal of Genetics and Plant Breeding (The)
SCOPUSWeb of Science
  • Year: 2014
  • Volume: 74
  • Issue: 3

Evaluation of doubled haploid culture conditions and regeneration of an indica rice hybrid

  • Author:
  • Anjanabha Bhattacharya, Venugopal Mikkilineni, Laxmikant Verma, Bhavesh Palan, Kishor Mali, Bharat Char
  • Total Page Count: 3
  • Page Number: 384 to 386

Mahyco Research Centre, Dawalwadi, Maharashtra Hybrid Seeds Company Limited, Aurangabad Road, Jalna-431203, Maharashtra

*Corresponding author's e-mail: anjanabha.bhattacharya@mahyco.com

Online published on 28 August, 2014.

Abstract

A study was undertaken to develop an effective protocol to generate doubled haploid plants from a proprietary Mahyco hybrid MRP5401. A total of 20,300 anthers were plated on four different DH media (basal N6 + phytohormones) and obtained a callus induction success rate of 1.52%, of which 0.48% developed into green plants. A total of 232 lines were obtained of which, 98 lines showed homozygosity in DH2–3 generations. Well established DH lines from MRP5401 in DH3 generation had early flowering and yield gain.

Keywords

Rice, doubled haploid (DH), anther culture, hybrid, callus induction, plant regeneration, genetic analysis