Indian Journal of Genetics and Plant Breeding (The)
SCOPUSWeb of Science
  • Year: 2015
  • Volume: 75
  • Issue: 4

An improved in vitro germination medium for recalcitrant bread wheat (Triticum aestivum L.) pollen

  • Author:
  • P. Jayaprakash, S. Annapoorani, V. K. Vikas, M. Sivasamy, J. Kumar1, K. Saravannan2, E. Punniakotti, D. Sheeba
  • Total Page Count: 7
  • Page Number: 446 to 452

1ICAR-IARI Regional Station, Shimla, Himachal Pradesh

2Department of Genetics and Plant Breeding, Faculty of Agriculture, Annamalai University, Tamil Nadu

ICAR-IARI, Regional Station, Wellington, The Nilgiris, 643 231, Tamil Nadu

*Corresponding author's e-mail: jpsarit@gmail.com

Online published on 9 November, 2015.

Abstract

There is no robust and quick technique available for testing germination of wheat pollen. In this study, Brewbaker and Kwack (BK) pollen germination medium (PGM) was adjusted with-amino caproic acid (EACA), peptone water and maltose to develop an improved medium for wheat. Germination of pollen in this PGM ranged from 92% to 98.2% depending upon genotype pollen germination media (PGM). This is the first report of PGM which has >95% wheat pollen germination in vitro.

Keywords

Recalcitrant wheat pollen, peptone water, maltose, €-amino caproic acid (EACA), in vitro pollen germination