1Department of Plant Pathology, Tirtash Tobacco Research Institute, Behshahr, Mazandaran, Iran
2Department of Plant Breeding and Biotechnology, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Mazandaran, Iran
3Department of Plant Breeding and Biotechnology, Institute of Soil Science and Plant Cultivation, State Research Institute, ul. Czartoryskich 8, PL-24-100, Pulawy, Poland
Department of Plant Biotechnology, Tirtash Tobacco Research Institute, Behshahr, Mazandaran, Iran
*Corresponding author's e-mail: d.soorni@gmail.com
Online published on 1 October, 2016.
Viral diseases such as Potato Virus Y (PVY) in tobacco plants can cause a lot of damage annually. In order to obtain PVY resistant doubled haploids (DH) of Nicotiana tabacum with desirable traits. Two varieties of tobacco VAM resistant to PVY and K326 of recognized high quality of leaves were crossed. The F1 hybrids were grown in pots and the anthers were cultured on PGR-free MS medium. After production of somatic embryos, colchicine treatment (0.4%) was applied for 8 hours. Thereafter, 184 plantlets were transferred to greenhouse and then moved to the field and self-pollinated. The derivatives of colchicine treated and fertile plants were inoculated with PVY0. DAS-ELISA technique and rating of symptoms were used to select resistant plants. From a total of seventeen DH, seven plants were selected as pure lines and evaluated under field condition. The DH lines showed significant differences between them for morphological and chemical traits. Some of the DHs had longer and wider leaves than K326 cultivar as control. The content of sugars was not affected by genotype. Finally, three doubled haploid lines (TDH-03, TDH-09, TDH-11) with desirable characteristics were selected as PVY resistance.
Tobacco breeding, doubled haploid, potato virus Y, ELISA, GGE biplot
