Indian Journal of Genetics and Plant Breeding (The)
SCOPUSWeb of Science
  • Year: 2017
  • Volume: 77
  • Issue: 3

Fertility restorer (Rf) gene linked STMS marker: An efficient tool for testing the genetic purity of hybrid rice (Oryza sativa L.) seed

  • Author:
  • Praveen K. Singh, Shiv K. Yadav, Chandra Prakash1, Sanjay Singh1, S. K. Chakrabarty, N. K. Singh1
  • Total Page Count: 4
  • Page Number: 422 to 425

1ICAR-National Research Centre on Plant Biotechnology, New Delhi-110012

Division of Seed Science and Technology, ICAR-Indian Agricultural research Institute, New Delhi-110 012

*Corresponding author's e-mail: Praveen.aqrbhu@gmail.com

Online published on 12 September, 2017.

Abstract

A fertility restorer (Rf) gene linked STMS marker, RM 6100 as well as Grow Out Test (GOT) were used for testing the genetic purity of hybrid seed of eight popular rice hybrids. The genetic purity ranging from 96.00% to 100.00%, could be determined through marker, whereas, it varied from 97.00% to 99.50% in GOT. The comparative assessment of both methods exhibited that molecular marker was more efficient than GOT, as it could detect 0.25–2.50% more impurities very precisely as compared to GOT. It suggests that a single Rf gene linked co-dominant STMS marker appeared to be a simple, precise and quick option over GOT for testing the purity of hybrid seed lots in short span of time.

Keywords

STMS marker, fertility restorer gene, rice, genetic purity, hybrid