Selection of reliable reference genes for gene expression studies by quantitative real-time PCR in Anoectochilus roxburghii

RT-qPCR is a sensitive, efficient and reliable method for gene expression studies and reference gene expression stability was essential for RT-qPCR. Since previous studies showed that no reference gene could exhibit changeless expression pattern in all experiments, this study determined the gene expression stability of 11 reference genes across various treatments in Anoectochilus roxburghii. The results indicated that expression stability of genes varied considerably under different treatments. The genes, GAPDH, EF1α and ACT1 emerged as the most reliable reference genes.