Department of Botany, RTM Nagpur University, MJP Educational Campus, Amravati Road, Nagpur440 033
*Corresponding author's e-mail: rsbadere@nagpuruniversity.nic.in
Online published on 28 October, 2020.
The shoot-tip explant harvested from ethyl methanesulphonate (EMS) and gamma ray (GR) mutagenized seedling was cultured over MS medium fortified with NAA and BAP for five generations to amplify the mutated sector. Mutagens reduced the regeneration efficiency of the explant and affected its plant growth regulator-dependence for multiple shoot induction. While the 12d-old shoot-tip from GR-treated seedling induced shoots with 0.5μM NAA+6.6μM BAP; that from EMS-treated seedling induced shoots with 8.8μM BAP. The present study establishes that the mutagens affect the regeneration process in the explant.
EMS, Gamma ray, In vitro mutagenesis, Plant growth regulator
