1Regional Agricultural Research Station, Professor Jayashanakar Telangana State Agricultural University (PJTSAU), Palem509 215, Nagarkurnool Dist., Telangana
2International Crops Research Institute for Semi-Arid Tropics (ICRISAT), HyderabadTelangana
3ICAR-National Bureau of Plant Genetic Resources, Regional Station, Rajendranagar500 030, Hyderabad, Telangana
4Regional Agricultural Research Station, PJTSAU, Warangal, Telangana
College of Horticulture, Sri Konda Laxman Telangana State Horticultural University (SKLTSHU), Rajendranagar500 030, Hyderabad, Telangana
*Corresponding author's e-mail: saidu_genetics@yahoo.co.in
**Present address: College of Horticulture, SKLTSHU, Mojerla509 382, Telangana
Online published on 16 August, 2021.
In this study, 29 genotypes of (Lablab purpureus) Indian bean augmented from different geographical regions were analyzed for genetic diversity using 39 RAPDs. The binary matrix data revealed 97.4% polymorphism among genotypes, with 2322 amplification fragments. The PIC values ranged from 0.25 to 0.96 with an average of 0.70. Markers OPA05, OPB15, and OPV12 showed high discrimination among all the genotypes. A magnificent range of Jaccard's genetic similarity coefficient was evaluated and UPGMA based dendrogram classified genotypes into 4 major clusters. IC-384066 and NSJ/NAIP/192 were found to be unique and highly diversified. The same grouping patterns were also observed in the model-based STRUCTURE analysis (K=2). The study showed the degree and distribution of genetic diversity in Dolichos bean, which can be utilized to identify the parental lines, to develop mapping populations and breeding.
Dolichos bean, Genetic diversity, RAPD, Structure, Upgma
