Indian Journal of Genetics and Plant Breeding (The)
SCOPUSWeb of Science
  • Year: 2022
  • Volume: 82
  • Issue: 1

Genetic diversity and population structure of Indian willow (Salix tetrasperma Roxb.) with dominant molecular markers along its distribution range in Himalayan region

  • Author:
  • J. P. Sharma, Sanjeev Thakur, H. P. Sankhyan, S. K. Jha2, Rajnish Sharma1, Poonam Kanwar1, Vikrant Gautam3, Neeraj Sankhyan4
  • Total Page Count: 8
  • Page Number: 73 to 80

1Department of Biotechnology, UHF, Nauni-173 230, Solan, Himachal Pradesh, India

2Department of Forest Biology and Tree Improvement, College of Forestry, Navsari Agriculture University, Navsari, Gujarat, India

3ICAR-IARI Regional Station, Amartara Cottage, Shimla, Himachal Pradesh, India

4Department of Basic Sciences, UHF, Nauni-173 230, Solan, Himachal Pradesh, India

Department of Tree Improvement and Genetic Resources, Dr YS Parmar University of Horticulture and Forestry

*Corresponding Author: Author, Department of Tree Improvement and Genetic Resources, Dr YS Parmar University of Horticulture and Forestry, Nauni-173 230, Solan, Himachal Pradesh, India, E-Mail: jptigr@yspuniversity.ac.in

Online published on 19 May, 2022.

Abstract

Indian willow (Salix tetrasperma) is an agriculturally useful tree which occurs over a wide geographic area across South Asia and bears importance. So far, this species has never been studied for molecular genetic diversity. The present study was, therefore, carried out to assess the genetic diversity and population structure analysis using RAPD and ISSR molecular markers in diverse genotypes from five populations covering North India. The mean number of effective alleles, Shannon information index and gene diversity i.e., 1.38 ± 0.013, 0.35 ± 0.010 and 0.23 ± 0.007, respectively were obtained with RAPD + ISSR markers. The analysis of molecular variance generated by RAPD + ISSR revealed a higher genetic variation (87%) within population as compared to that of among population (13%). Nei genetic distance was maximum (0.185) between Jammu and Kashmir and Punjab populations. Significant Mantel correlation (r=0.551,) was obtained between RAPD and ISSR markers. Bayesian clustering pattern obtained through STRUCTURE software showed four gene pools. Based on the genetic information obtained with regards to Indian willow by combining the RAPD and ISSR marker systems, it is proposed that an individual tree be selected within populations rather than among populations for the improvement of economic traits of Indian willow alongwith conservation of entire ecological populations.

Keywords

Differentiation, Genetic, ISSR, Mantel correlation, Nei genetic distance