1Department of Plant Breeding and Genetics, College of Agriculture, Jawaharlal Nehru Krishi Vishwa Vidyalaya (JNKVV), Jabalpur-482 004, Madhya Pradesh, India
2College of Agriculture-Warseoni, JNKVV, Jabalpur-481 331, India
Biotechnology Centre, JNKVV, Jabalpur
*Corresponding Author: Radheshyam Sharma, Biotechnology Centre, College of Agriculture, Jawaharlal Nehru Krishi Vishwa Vidyalaya (JNKVV), Jabalpur-482 004, Madhya Pradesh, India, E-Mail: radhebiotech88@gmail.com
Online published on 19 May, 2022.
A protocol was developed for organogenesis and in vitro multiplication of chironji using young leaf and nodal segments. Maximum 41 callus were induced in MS containing 2.5mg/l 2,4-D after 3 weeks of inoculations of leaf explants. Further, callus and nodal segments were inoculated alone and combination of Thidiazuron (TDZ), BAP, and Kinetin and the maximum shoot induction was obtained in a wood plant medium 2.5mg/L TDZ alone enriched with 0.1% of activated charcoal. In comparison, the maximum shoot proliferation (78%) was observed in ½WPM containing 2.5mg/L TDZ and 0.5mg/L GA3 with 5.7 shoots per explants. Maximum 8 roots were observed in vitro regenerated shoots with WPM supplemented with 2.0 mg/l IBA enriched with 0.2% activated charcoal. Plants with 3-4cm in root length were acclimatized and transferred to pots containing an autoclaved mixture of soil, sand and manure in 2:1:1 ratio. Approximately 70% survival rate was recorded from in vitro grown plantlets on transfer to pots.
Callus, Chironji, Hardening, Nodal segments, Shoot induction, Root induction
