Indian Journal of Genetics and Plant Breeding (The)
SCOPUSWeb of Science
  • Year: 2022
  • Volume: 82
  • Issue: 2

Isolation and characterization of novel gene TaSSRP differentially expressed in wheat (Triticum aestivum L.) genotypes under heat stress

  • Author:
  • Mawuli K. Azameti1, P. K. Singh1, Kishor Gaikwad, Monika Dalal, Ajay Arora2, Vandana Rai, Jasdeep C. Padaria*
  • Total Page Count: 3
  • Page Number: 224 to 226

1Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110 012, India

2Division of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi, 110 012, India

ICAR-National Institute for Plant Biotechnology, New Delhi, 110 012, India

*Corresponding Author: Jasdeep C. Padaria, ICAR-National Institute for Plant Biotechnology, New Delhi, 110 012, India, E-Mail: jasdeep_kaur64@yahoo.co.in

Online published on 14 February, 2023.

Abstract

Analysis of heat stress-responsive transcriptome data in wheat genotype Raj 3765 revealed that expression of salt stress root protein (SSRP) RS1 was observed to increase by 8.29 folds in the flag leaf at the grain filling stage. Further validation of the gene under heat stress condition (42°C for 6 hours) by qRT-PCR revealed an increase of 8.42 folds. In-silico studies revealed that the gene is localized mainly in the cytoplasm, and did not contain transmembrane helix. The presence of 15 phosphorylation sites as indicated by in-silico analysis could play significant roles in the heat stress tolerance.

Keywords

Heat stress tolerance, In-silico, TaSSRP, Wheat