1Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad, 500 030, Telangana, India
2Acharya N.G. Ranga Agricultural University, Lam, Guntur, 522 034, Andhra Pradesh, India
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad, 500 030, Telangana, India
*Corresponding Author: S. Senthilvel, ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad, 500 030, Telangana, India, E-Mail: senthilvel.senapathy@icar.gov.in
Online published on 16 February, 2023.
Castor is an industrially important oilseed crop, which provides raw material to many industries. Despite its industrial importance, the genomic resources available for molecular breeding applications are limited. In the present study, a set of 135 SNP loci polymorphic between two castor inbred lines namely, JC-12 and RG-1963 were targeted to develop competitive allele specific PCR assays for genotyping purposes. The assays were validated in the F2 population of JC-12 × RG-1963 cross. Out of 135 assays designed, 124 were found to be successful in discriminating three expected genotypes in the F2 population. Using the SNP genotyping data, a genetic linkage map representing 10 haploid chromosomes of castor was constructed, which corresponded very well with the physical map. This set of validated SNP markers is a useful resource for application in molecular breeding of castor.
Ricinus communis, KASP assay, Molecular markers, Linkage map
