Advanced Applied Sciences Research Group, Dong Nai Technology University, Bien Hoa City, 76100, Vietnam
1Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD, Australia
2Faculty of Applied Sciences, Ton Duc Thang University, 19 Nguyen Huu tho, Tan Phong, District 7, Ho Chi Minh City, 700000, Vietnam
Indica rice variety, IR50404 is the most-grown rice in Vietnam’s Mekong Delta. It is susceptible to multiple blast diseases, making it an ideal genotype for identifying pathological trait mutations. Chemically induced mutant lines were derived from IR50404 by treating seeds with ethyl methanesulfonate (EMS) (0.1 and 0.125%), methyl salicylate (MeSA) (0.1 and 0.125 mM), and their combinations. Chlorophyll, proline, and protease enzyme inhibitors were examined for disease tolerance in mutant rice-based plants. Blast resistance (41.6%) and protease inhibition (30.4%) were highest in T5 (0.1% EMS + 0.1 mM MeSA). Proline (3.91 g/mL) and chlorophyll (8.52 g/mL) concentrations were significantly higher after 0.125% EMS treatment than in the untreated control.In addition, a high rate of protease inhibition (30.4%) was improved after treatment with 0.125% EMS + 0.125 mM MeSA. Results showed that blast resistance was improved by 0.1% EMS and MeSA.This also has implications for the breeding of plant varieties other than rice.
Blast disease, Chlorophyll a and b, EMS, MeSA, Proline, Protease
