Indian Journal of Genetics and Plant Breeding (The)

SCOPUSWeb of Science
  • Year: 2024
  • Volume: 84
  • Issue: 4

Validation of molecular markers for the identification of resistant sources against white rust disease of rapeseed mustard caused by Albugo candida

  • Author:
  • Pooja Upadhyay, A. K. Tewari1,*, Usha Pant2, Prashant Vikram, Nitesh Singh, H. Rajashekara3
  • Total Page Count: 11
  • Page Number: 686 to 696

Faculty of Agricultural Science, SGT University, Gurugram, 122 505, Haryana, India

1Department of Plant Pathology, GBPUA&T, Pantnagar, 263 145, Uttarakhand, India

2Department of Genetics and Plant Breeding, GBPUA&T, Pantnagar, 263 145, Uttarakhand, India

3ICAR-Directorate of Cashew Research, Puttur, 574 202, Karnataka, India

Abstract

White rust or white blister disease caused by oomycete fungi, [Albugo candida (Persoon) Roussal] is one of the major devastating fungal diseases of rapeseed (Brassica napus) mustard. The continuous emergence of new pathogenic races is responsible for the breaking down of the resistance in already existing resistant cultivars. So, for finding new resistant sources against the pathogen, molecular markers have become new tools. In the present study, validation of 29 simple sequence repeat (SSR) markers was done to know the presence or absence of resistance (R) genes against selected Brassica genotypes using PCR analysis. Marker trait association analysis and multiple regression analysis indicated a significant association of six markers, namely, At5g41560, Ni2BO3, BrgMS329, MB5, BRMS-017 and NI-F02a with the immune, highly resistant and moderately resistant Brassica cultivars. These molecular markers may be utilized for the identification of resistant genotypes against A. candida pathogens in rapeseed mustard.

Keywords

White rust, Molecular makers, Resistance, Association analysis, Albugo candida