1The University of Transdisciplinary Health Sciences and Technology, Bengaluru560 064, Karnataka, India.
2Department of Biotechnology and Crop Improvement, Kittur Rani Chennamma College of Horticulture, Arabhavi591 218, University of Horticultural Sciences, Bagalkot, Karnataka, India.
$Present address Department of Biotechnology and Crop Improvement, Kittur Rani Chennamma College of Horticulture, Arabhavi591 218, University of Horticultural Sciences, Bagalkot, Karnataka, India.
4Silviculture and Forest Management Division, Institute of Wood Science and Technology (IWST), Malleshwaram, Bangalore560 003, Karnataka, India.
*Corresponding Author: P. Mohana Kumara, Department of Biotechnology and Crop Improvement, Kittur Rani Chennamma College of Horticulture, Arabhavi591 218, University of Horticultural Sciences, Bagalkot, India, E-Mail: monapatelpgatti@gmail.com
Pterocarpus dalbergioides and P. marsupium are deciduous tropical trees in the Fabaceae family, with P. dalbergioides native to India’s Andaman Islands and P. marsupium found in various Asian countries. This research investigated the applicability of specific primers for P. santalinus across these species. DNA isolation and PCR amplification were performed on 15 samples from each species. Out of 33 primers, 7 markers were amplified in P. dalbergioides and 15 in P. marsupium, yielding transferability rates of 21 and 33%, respectively. For P. dalbergioides, the effective population size (Ne) was 3.82 ± 1.84, observed heterozygosity (He) 0.42 ± 0.15, and allelic richness (Pa) 5.29 ± 2.01. In P. marsupium, Ne was 4.83 ± 0.91, He was 0.56 ± 0.10, and Pa was 7.07 ± 1.27. The average polymorphic information content (PIC) was 0.49 for P. dalbergioides and 0.54 for P. marsupium. The study underscores the potential of transferred SSR markers to assess genetic diversity in Pterocarpus species.
Genomic SSRs, cross transferability, polymorphism, tree species, Pterocarpus
