Resistance gene analogue assisted screening for mung bean yellow mosaic virus in mung bean (Vigna radiata L. Wilczek) genotypes

Mung bean production is severely constrained by mung bean yellow mosaic virus (MYMV) disease, making host resistance a priority for sustainable crop improvement. Resistance gene analogue (RGA) primers serve as effective molecular tools for identifying MYMV- resistant genotypes. The present study was carried out to screen 302 mung bean genotypes using RGA primers to identify resistant genotypes and validate their field performance under hotspot conditions. Among the four tested RGA primers, only two primers viz., CYR-1 (1236 bp) and VMR-1 (445bp) were successful in amplifying MYMV resistance related sequences. Among 25 genotypes, 13 were amplified by CYR-1 and 24 genotypes were amplified by VMR-1. Further, thirteen genotypes showed amplification for both the primers in common, indicating the presence of resistance genes for both MYMV and mung bean yellow mosaic India virus (MYMIV). These results were confirmed by t test indicating significant (p = 0.001) association of these two markers with MYMV disease in mung bean. The findings highlight the efficiency of CYR-1 and VMR-1 primers as reliable markers for screening MYMV resistance. Their integration into marker-assisted selection (MAS) can accelerate breeding programs aimed at developing resistant mung bean varieties.