Tissue culture system for Fraxinus velutina Toor

This study established an efficient in-vitro regeneration system for Fraxinus velutinaTorr. Embryonic axes and cotyledons were identified as the optimal explants. The highest callus induction rate of 98.43% was achieved using MS medium supplemented with 0.5 mg/L TDZ and 0.25 mg/L 6-BA (30 g/L sucrose, 8 g/L agar). For cotyledon explants, this same medium produced a 92.54% induction rate. The optimal adventitious shoot differentiation medium consisted of MS with 2 mg/L TDZ and 0.5 mg/L 6-BA, yielding a 90.28% differentiation rate. Rooting was most successful with 96.4% on MS medium containing 0.5 mg/L NAA. Transplanted seedlings showed a 97.8% survival rate. These findings provide technical support for large-scale propagation and genetic transformation of F. velutina.