Indian Journal of Genetics and Plant Breeding (The)
SCOPUSWeb of Science
  • Year: 2026
  • Volume: 85
  • Issue: 4Supplementary

Chromosome identification and karyotype analysis of rice bean [Vigna umbellata (Thunb.) Ohwi et Ohashi] using fluorescence in situ hybridization

  • Author:
  • Pooja Pathania1, Dhammaprakash P. Wankhede1, Gayacharan1, Rajkumar Subramani1*
  • Total Page Count: 3
  • Page Number: 820 to 822

1Division of Genomic Resources, ICAR- National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi110 012, India

*Corresponding Author: Rajkumar Subramani, Division of Genomic Resources, ICAR- National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi110 012, India, E-Mail: s.rajkumar@icar.org.in

Abstract

Rice bean (Vigna umbellata), an underutilized climate-resilient legume, includes the high-yielding, leaf spot resistance variety VRB-3 (Him Shakti). In the present study, using fluorescence in situ hybridization, one pair each of 45S and 5S rRNA gene loci was localized to the terminal region of the chromosomes and the estimated genome content for VRB-3 is 1.35pg against the reference standard Solanum lycopersicum cv. Stupicke.

Keywords

Flow cytometry, fluorescence in-situ hybridization, genome size, Vigna umbellata