In vitro raising of nucellar embryo derived seedlings for virus elimination in citrus

Plant regeneration was achieved from isolated nucellar embryos of C. jambhiri, C. reticulata cv. Cleopatra and Pectinifera; C. sinensis cvs. Blood Red, Jaffa and Mosambi and C. nobilis × C. deliciosa cv. Kinnow on MS medium supplemented with kinetin (0.2 mg/l) and sucrose (2%). The nucellars did not exhibit any variation in leaf morphology and isoperoxidases. However, the hybrids displayed differences for leaf shape and number of isoperoxidases as compared to nucellars.