Cytogenetics and Biotechnology Laboratory, Department of Botany, Osmania University, Hyderabad - 500 007
*Tissue Culture Laboratory, Agricultural Research Institute, APAU, Rajendranagar, Hyderabad 500 030
Healthy flowering plants have been regenerated in vitro from excised immature embryo ex-plants of Rosa hybrida cv. King's Ransom. The embryos developed into a callus mass when cultured on MS media with various concentrations and combinations of NAA and BAP. The combination of BAP produced organogenic callus and the specific combination of NAA 0.25 mg/l and BAP 1.0 mg/l induced an earlier development of adventitious shoots from the callus. The age of the embryo also plays a very important role in plantlet regeneration response. Four and five week old embryos (after flower opening) responded very favourably by producing maximum callus and shoots. The protocol developed presently for efficient plantlet regeneration from embryos can be adopted favourably for gene transfer experiments in Rosa hybrida cv. King's Ransom.
