Indian Journal of Horticulture
  • Year: 2001
  • Volume: 58
  • Issue: 3

In vitro plant regeneration in sweet orange (Citrus sinensis L. Osbeck.) cv. Mosambi and Jaffa

  • Author:
  • Krishan Kumar, A.S. Dhatt, M.I.S. Gill
  • Total Page Count: 4
  • Page Number: 208 to 211

Department of Horticulture, Punjab Agricultural University, Ludhiana 141 004

* Present address: Director, Punjab Horticultural Postharvest Technology Centre, Punjab Agricultural University, Ludhiana 141 004.

Abstract

Studies were conducted with sweet orange (Citrus sinensis L. Osbeck.) cv. Mosambi and Jaffa using epicotyl segments from in vitro grown nucellar seedlings. BAP (up to 1 mg/litre) was found optimum. However in Jaffa, maximum shoot proliferation was observed with BAP (2.0 mg/litre). Root development was maximum at the concentration of NAA (0.5 mg/litre) + IBA (0.5–1.0 mg/litre). However, root development was also better at NAA (1.0 mg/litre) alone. The complete plantlets were obtained with a survival rate of 66–67%.