Department of Horticulture, Punjab Agricultural University, Ludhiana 141 004
* Director, Horticultural Post-harvest Technology Centre, Punjab Agricultural University, Ludhiana 141 004
The experiment was conducted on Kinnow mandarins using epicotyl segments obtained from in-vitro grown seedlings. Among the different levels of BAP evaluated, maximum mean shoot proliferation, shoot number, shoot length and leaf number were observed in BAP (1 mg/litre). This shows the optimum level of BAP for morphogenic response in Kinnow mandarins. The root development was better in concentrations of 0.5–1.0 mg/litre IBA + 0.5 mg/litre NAA. The complete plantlets were obtained with a survival rate of 68.8%. This is the first report on in-vitro plant regeneration of Kinnow mandarins via direct organogenesis obtained through epicotyl segments from in-vitro grown seedlings.
