Biotechnology Laboratory, Division of Horticulture, ICAR Research Complex for NEH Region, Barapani, Meghalaya 793 103
* Central Plantation Crops Resarch Institute, Kasaragod 671 124, and NRC (Orchids), Sikkim respectively
** Central Plantation Crops Resarch Institute, Kasaragod 671 124, and NRC (Orchids), Sikkim respectively
The studies were undertaken to find out optimum concentration of BAP using quadratic model. Shoots (0.5–1.0 cm long) from in-vitro proliferated plants of 5 Citrus species, viz. C. madurensis, C. volkameriana, C. sinensis, C. limon and Poncirns trifoliata × C. sinensis (Troyer citrange) were cultured in MS supplemented with varying concentrations of BAP and kinetin. Morphological observations recorded 4 weeks after culture initiation indicated that there was a significant interaction between species and cytokinins, and species and concentration. Among species C. volkameriana recorded highest plant weight, while C. limon recorded lowest plant weight. BAP was a better cytokinin for shoot proliferation, whereas kinetin for producing a fewer but healthier plantlets. The optimum concentration of BAP for producing maximum shoots was worked out using quadratic response equation. Troyer citrange responded to a high level of BAP, while C. sinensis (malta) responded to a relatively low level of BAP.
