Department of Horticulture, Annamalai University, Annamalainagar 608 002
Micropropagation studies were carried out to standardize the type of explant and level of plant growth regulators in papaya. Different explants like shoot tip, leaf bit and nodal segment were used to induce callus in MS media supplemented with BA and IAA at different concentrations. Shoot tip recorded highest callusability per cent within 25 days in MS medium supplemented with 1.0 mg/litre IAA and 4.5 mg/litre BA. Development of shoots was observed from shoot tip callus cultured on MS medium supplemented with BA 4.5 mg/litre compared to kinetin. Among the auxins tried, IAA 1.0 mg/litre was the best for rhizogenesis.
