Division of Floriculture and Landscaping, Indian Agricultural Research Institute, New Delhi 710 012
* NRC on Plant Biotechnology, IARI, New Delhi 110 012
Mutants of carnation cv. Espana induced through in-vitro mutagenesis using physidal (gamma rays) and chemical nitroso-methyl urea and ethyl methane sulphonate mutagens were categorised into five groups on the basis of morphological characteristics especially, flower colour variation. Further, these mutants along with original cultivar were subjected to electrophoretic analysis of total soluble proteins. All the individuals showed differences among themselves through banding pattern of soluble proteins. Band intensity of soluble proteins in all the variants varied at most of the loci among themselves and as compared to parental cultivar, though similar bands were also obtained at few loci. Number of bands also differed in mutant 4 and 5 as compared to others. The differences in banding pattern of soluble proteins reflected the variability in mutants at genetic level.
