Use of random amplified polymorphic DNA (RAPD) analysis to confirm genetic stability of in vitro regenerated grape plantlets

In commercial mlcropropagation, it is imperative to certify the plantlets for the genetic uniformity during different multiplication stages. Randomly Amplified Polymorphic DNA (RAPD) analyses of in vitro cloned Pusa Urvashi plantlets with the field-grown mother plant and other genotypes was carried out. Out of twenty decamer primers used for the study, fifteen were found polymorphic; while five were monomorphic for the three grape genotypes, viz. Pusa Urvashi, Pusa Navrang and Centennial Seedless. Primers were selected on the basis of their amplification product for clear and better interpretation. The in vitro raised plantlets and the mother plant showed the monomorphic bands confirming thegenetic homogeneity in the tissue cultured plantlets. Present study supports the fact that the grape plantlets regenerated from the nodal segment and multiplied using repetitive subculture of two-node micro-cuttings, did not exhibit any induced variations and were largely stable and true-to-the-type.