1Department of Biotechnology, Bundelkhand University, Jhansi 284 128
2Horticulture Commissioner, Government of India, New Delhi 110 001
*Corresponding author’s E-mail: kvprasad66@yahoo.co.in
DNA fingerprinting of twenty-one Indian bred rose (Rosa spp.) cultivars as done by Random Amplified Polymorphic DNA (RAPD) to assess the polymorphism and genetic relatedness among the selected cultivars. Forty random decamer primers were used to analyze the polymorphism among the cultivars by comparing the DNA banding patterns as revealed by agarose gel electrophoresis. Out of 40 random primers used, 18 primers amplified the scorable banding patterns. These 18 primers gave 113 polymorphic bands out of 131 bands analyzed. The percentage polymorphism ranged from 66 to 100. The polymorphic bands were in the range of 0.40 to 2.4 kb. The dendogram obtained by Jaccard’s similarity coefficient method distributed 21 Indian bred rose cultivars into four major clusters. The study clearly indicated that RAPD marker technique could be employed for rapid fingerprinting of rose cultivars and for genetic identification and patent protection purposes.
Random Amplified Polymorphic DNA analysis, random primers, Indian roses, genetic fingerprinting
