Tissue Culture Laboratory, Department of Botany, Gauhati University, Guwahati 781 014.
*Corresponding author's E-mail: devipapori@yahoo.com
**Present address: Department of Botany, Mizoram University, Aizwal 796 012.
Procedures were developed for micropropagation of Bacopa monneiri (L.) Penn. by culturing leaf explants in Murashige and Skoog medium. Nodal segments of Bacopa were first cultured on hormone-free medium for five days. Then leaves from disease/fungus free shoots were used as explants in different combinations of PGRs in MS medium. PGRs used were kinetin and NAA, and also kinetin, NAA and IBA in combinations. Better results in terms of plantlet production and time required for generation were observed in MS medium with 0.1 µg/ml kinetin plus 0.5 µg/ml NAA. Addition of IBA exhibited variation in results and best results were obtained in 0.1 µg/ml of kinetin, 0.5 µg/ml of NAA and l.0 µg/ml of IBA.
Bacopa monneiri, micropropagation, leaf explant
