In vitro shoot regeneration in nematode tolerant grape rootstock 1613C

In vitro shoot regeneration studies were carried out in rootstock 1613 C (Vitis solonis x V. labrusca var. Othello) using nodal segment and shoot tip as the explants. The MS medium supplemented with BAP and kinetin in different combinations and concentrations were used for shoot regeneration and proliferation. Maximum explant establishment was achieved when nodal segments were cultured during April. The maximum shoot regeneration in nodal segment was obtained on MS medium supplemented with BAP (1 mgl⁻¹) + kin (1 mgl⁻¹), while in shoot tips, it was obtained on MS medium supplemented with BAP (1 mgl⁻¹) + kin (0.5 mgl⁻¹). The established shoots were proliferated on MS medium supplemented with BAP (1 mgl⁻¹) + kin (1 mg l⁻¹).