Genetic diversity studies on polyembryonic and monoembryonic mango genotypes using molecular markers

Twelve polyembryonic and ten monoembryonic mango genotypes were examined using random amplified polymorphic DNA (RAPD) markers with 50 decamer primers of arbitrary sequence. Out of the 50 primers screened, 30 were informative and showed good amplification of mango DNA. Cluster analysis based on the RAPD markers produced a dendrogram of the genetic relatedness among the 22 polyembryonic and monoembryonic genotypes. The major bifurcations in the dendrogram separated the genotypes based on their embryo type. The grouping indicates that monoembryonic and polyembryonic genotypes have a different genetic base with different geographical origin. Among the monoembryonic genotypes, Sensation and Tommy Atkins were quite dose based on their common geographical origin. The monoembryonic hybrid, Pusa Arunima developed at IARI, New Delhi formed a unique cluster, while the polyembryonic genotype Starch did not group with any other and formed a distinct cluster. Results suggested the highly heterozygous nature of different genotypes.