Division of Fruits and Horticultural Technology, Indian Agricultural Research Institute, New Delhi, 110 012.
*Corresponding author's: E-mail: aksingh36@yahoo.com
1NRC on Plant Biotechnology, I.A.R.I., New Delhi, 110 012
Genetic diversity was estimated in 32 accessions from different ecogeographical regions of India representing Vitis vinifera and V. champini. Twenty pre-selected SSR primers generated a total of 64 easily scorable amplification products. Out of total 64 reproducible fragments generated, 60 were (93.7%) polymorphic. The cluster analysis indicated that grape germplasm in India contributes broad genetic base with the values of genetic similarity coefficient ranging from 0.48 to 0.86. UPGMA analysis resulted in the formation of two main clusters, consisting mainly of commercial cultivars (Vitis vinifera) and rootstock (Vitis champini). The Vitis vinifera group was more heterogenous and it further divided in sub clusters. Two varieties belonging to Vitis vinifera grouped with Vitis champini. All other varieties were differentiated by unique pattern with 20 SSR markers. The high discriminating power of the loci suggests that a selection of the most robust SSR markers may be able to differentiate commercial cultivars, rootstocks or hybrids.The results showed that SSR is an efficient and reliable marker system for genetic analysis of grape cultivars.
Grape, Vitis sp., molecular marker, SSR marker, genetic characterization
