Indian Journal of Horticulture

  • Year: 2010
  • Volume: 64
  • Issue: 4

Indirect organogenesis in ginger

  • Author:
  • Resmi Paul, M.R. Shylaja
  • Total Page Count: 5
  • DOI:
  • Page Number: 513 to 517

Department of Plantation Crops and Spices, College of Horticulture, Kerala Agricultural University, 680 656.

Abstract

Indirect organogenesis was attempted in two ginger cultivars viz., Maran and Rio-de-Janeiro. Rhizome buds and various parts of in vitro adventitious bud regenerants viz., pseudostem, shoot tip, folded and unfolded leaves were used as explants. Shoot tip was identified as the best explant for callusing. Half-strength MS medium supplemented with 1.00 mg l−1 2,4-D recorded the highest callusing (56.37%) and callus growth (CI - 55.11). Half- strength MS medium supplemented with 3.0 mg l−1 BAP was found ideal for shoot morphogenesis (38.46%) and rooting.The cultivar Rio-de-Janeiro recorded higher callusing (44.01%), callus growth (CI - 38.59), early (26 days) and high shoot morphogenesis (39%) and high rate of shoot proliferation.The cultivar Maran was found better for root characters like number and length.

Keywords

Indirect organogenesis, ginger, shoot morphogenesis, somaclonal variation