In vitro plant regeneration in brinjal from cultured seedling explants

In vitro plant regeneration of brinjal genotype BL-3 was tried using hypocotyl, cotyledon and leaf explants from in vitro raised seedlings on Murashige and Skoog medium fortified with 6-benzylamino purine (BAP) and kinetin (kin) combination (2.0–3.0 mgl⁻¹ BAP with or without 1.0 mgl⁻¹ kin). the cotyledon explant gave cent percent regeneration on MS medium fortified with 2.0 mgl⁻¹ BAP, 2.5 mgl⁻¹ BAP, or 2.5 mgl⁻¹ BAP + 1.0 mgl⁻¹ kin, while the highest numbers of buds on 2.5 mgl⁻¹ BAP (24.90), followed by 2.0 mgl⁻¹ BAP (17.90). Leaf explant also induced cent percent regeneration on MS medium fortified with 2.0 mgl⁻¹ BAP and maximum number of buds (9.53) regenerated with 2.5 mgl⁻¹ BAP. hypocotyl had the maximum regeneration (66.53%) and maximum buds (3.96) on MS with 2.5 mgl⁻¹ BAP. Maximum bud elongation (58.73%) was obtained on ½ MS medium supplemented with 0.3 mgl⁻¹BAP + double agar. MS basal medium induced maximum rooting of 61.11% plantlets. the hardening with 0.2% bavistin solution enhanced the survival efficiency of plantlets to 81.81%. The plantlets were established in the polythene bags and then transferred to earthen pots in the glasshouse, where they grew, flowered and set fruits.