Department of Vegetables Crops, Punjab Agricultural University, Ludhiana, 141 004
*Corresponding author's E-mail: ajmerdhatt@gmail.com
**School of Agricultural Biotechnology, PAU, Ludhiana 141 004.
***IIHR, Bangalore 560 089.
Brinjal were regenerated from callus derived from hypocotyl, cotyledon and root explants of five genotypes, namely Punjab Barsati, Punjab Sadabahar, Jamuni Gola, PBSR-11 and BB-93C on MS medium containing different concentrations of IAA and BAP. A combination of 2.5 mg/l IAA + 0.5 mg/l BAP was found optimum for adventitious shoot induction from all explants. Genotype, explant and genotype x explant interaction showed highly significant effects on organogensis. Among genotypes, PBSR-11 showed maximum response for organogenesis (79.43%). However, among explants, cotyledon was significantly better than hypocotyl and root. Plants regenerated via adventitious shoots were rooted on half-strength MS basal medium in vitro.
Egg plant, plant regeneration, organogenesis, shoot induction
