Department of Molecular Biology and Genetic Engineering, Pantnagar, 263 145, Uttarakhand
*Corresponding author's present address: Department of Molecular Biology & Genetic Engineering, G.B. Pant University of Agri. & Tech. Pantnagar 263 145; E-mail: anilgaur123@rediffmail.com
**College of Basic Science and Humanities, G.B. Pant University of Agric. & Tech. Pantnagar 263 145
Small vegetative parts of Aconitum balfourii from different growth stages were excised for in vitro establishment. The callus induction was achieved from cultured leaf segment on MS medium fortified with NAA (2.5 mg/1) and BAP (1 mg/1). Calli upon transfer onto MS medium containing BA alone (0.5–4 mg/l) for shoot regeneration, multiplication and elongation. Up to 25 shoots per culture flask were obtained on 2 mg/l BAP after 7–8 weeks of culture. The optimum rooting on micro-shoots was observed on MS supplemented with IAA (1.8 mg/l). The in vitro developed plantlets with elongated roots were then subjected for further ex vitro rooting, acclimatization and hardening.
Aconitum balfourii, in vitro regeneration, micro-propagation
