Indian Journal of Horticulture
  • Year: 2012
  • Volume: 69
  • Issue: 1

Comparison of fluorescein- and 32P-labelled probes for safe detection of potato spindle tuber viroid in potato

  • Author:
  • Priyanka Kaundal, Jagesh K. Tiwari, Nimisha Kaushal, Deep Kumar, J. Gopal, S.K. Pandey, I.D. Garg
  • Total Page Count: 4
  • Page Number: 121 to 124

Central Potato Research Institute, Shimla 171 001, Himachal Pradesh

*Corresponding author's present address: Division of Crop Improvement, Central Potato Research Institute, Shimla 171 001; E-mail: jageshtiwari@gmail.com

Online published on 12 April, 2012.

Abstract

Two methods namely fluorescein- and 32P-labelled probes were tested for detection of Potato Spindle tuber viroid in potato. Out of 94 potato microplants tested, 82 were found positive, while 12 were virus-free. On comparison with NASH, it could be seen that fluorescein-labelled probe was quite cost effective.

Keywords

Fluorescein, 32P-labelled, probe, potato spindle tuber viroid