1Division of Vegetable Science, Indian Agricultural Research Institute, New Delhi
2Rajiv Gandhi Biotechnology Centre, RTM Nagpur University, Nagpur, 440001
Division of Genomic Resources, National Bureau of Plant Genetic Resources, New Delhi, 110012
*Corresponding author's E-mail: ambikabg@yahoo.co.in
Online published on 19 April, 2014.
We report on the identification of an inter-simple sequence repeat marker (ISSR) associated with the gynoecious trait in bitter gourd. Twenty-four gynoecious plants belonging to the F5 generation derived from a previously identified gynoecious line DBGy-201were screened using 200 RAPD and 28 ISSR markers. A 1000 base pair fragment specific to the gynoecious plants was amplified by the primer (AC)8T. Since bitter gourd flowers 35–40 days after sowing, identification of this marker associated with gynoecy is of great significance to ascertain purity of gynoecious lines at an early stage of development for a cost effective hybrid seed production.
Bitter gourd, Gynoecious-specific, ISSR marker
