1School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana
Department of Vegetable Science, Punjab Agricultural University, Ludhiana-141004
*Corresponding author's E-mail: mkaur97@rediffmail.com
Online published on 6 July, 2015.
Direct plant regeneration of three eggplant genotypes, viz., BL-5, BR-14 and BSR-23 was studied with hypocotyl, cotyledon and leaf explants on Murashige and Skoog (MS) medium fortified with different concentrations BAP and Kin. The interaction of genotype, medium and explant gave 80.36% regeneration from cotyledons of BL-5 on MS medium fortified with 3.0 mgl−1 BAP and 75.19% for cotyledons along with 3.0 mgl−1 BAP and 1.0 mgl−1 Kin. However, the same genotype induced 21.76 buds per cotyledon on MS medium supplemented with 2.5 mgl−1 BAP + 1.0 mgl−1 Kin. In general, maximum shoot elongation (%) was recorded in BSR-23 (58.53%) followed by BL-5 (56.16%), whereas, maximum rooting (%) was induced in BL-5 (57.50%), followed by BSR-23 (57.14%). The rooted plantlets were hardened on wet filter paper with 0.2% carbendazim solution for 20 days, transferred to polythene bags in greenhouse and thereafter, at 4–5 leaf stage to the earthen pots for growth and flowering.
Eggplant, hypocotyl, cotyledon, in vitro, regeneration, leaf
