Indian Journal of Horticulture
  • Year: 2015
  • Volume: 72
  • Issue: 2

RNA isolation from high polyphenol containing tissues of pomegranate

  • Author:
  • N.V. Singh, Thangasamy Saminathan1, Ram Chandra, Chandrakant Awachare2, K. Dhinesh Babu, D.M. Mundewadikar3, R.K. Pal
  • Total Page Count: 5
  • Page Number: 273 to 277

1Department of Biology, Gus R. Douglass Institute, West Virginia State Univ., WV, USA

2ICAR-Indian Institute of Horticultural Research, Bengaluru, Karnataka

3MGM's Institute of Biosciences and Technology, Aurangabad, Maharashtra

ICAR-National Research Centre on Pomegranate, NH-9, Solapur-Pune Highway, Kegaon (PO), Solapur-413 255, Maharashtra

*Corresponding author's E-mail: nripendras72@gmail.com

Online published on 6 July, 2015.

Abstract

Three methods of RNA isolation were evaluated based on quality and quantity of the RNA isolated. The methods used for RNA isolation were CTAB-LiCl method, Direct-zol™ RNA MiniPrep (ZymoResearch), and TRIzol® reagent (Life Tech.). RNA quality was assessed using spectrophotometric methods, agarose gel electrophoresis and Agilent 2100 Bioanalyzer. RNA isolation using modified CTAB-LiCl was found effective in extraction of sufficient quantity and high quality RNA from new leaves (617 μg/g FW), seedlings (636 μg/g FW) and flower buds (603 μg/g FW) having RNA integrity number (RIN) values of 8.5 each.

Keywords

Pomegranate, RNA isolation, RNA intergrity number