ICAR-Directorate of Onion & Garlic Research, Rajgurunagar, Pune-410 505, Maharashtra
1Div. of Vegetable Science, ICAR-IARI, New Delhi-110012
2Vice Chancellor, Dr BSKKV, Dapoli
A study was undertaken to determine the LD50 in garlic variety G-41, to create variability for desirable traits using sodium azide (SA), colchicine and ethyl methane sulphonate (EMS). Half cut garlic cloves after treating for 6 and 12 h, were planted in randomized block design. Among the durations of treatments, concentrations required to attain LD50 was higher when treated for 6 hours as compared to 12 h in all the three mutagens. LD50 in SA can be obtained at 0.021 and 0.024%, in colchicine at 0.058 and 0.097% and in EMS at 0.674 and 1.222% concentration in 12 h treatment, whereas, it can be obtained at 0.095 and 0.108% in SA, 0.124 and 0.145% in colchicine and 0.826 and 1.222% in EMS in 6 h treatment, respectively at the time of harvesting and at 45 days after planting. Same concentrations can be tried in other varieties or lines of garlic to create variability for further selection.
Colchicine, ethyl methane sulphonate, garlic, mutation, LD50, sodium azide
